Part:BBa_K3582205:Design

Inhibitory Sequence 1 Grafted In Cyclotide Kalata B1 for EPCR-PfEMP1 interaction

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 112
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 143
  Illegal BsaI.rc site found at 109
  

Design Notes

• 1] RFC10 criteria satisfied
• 2] Strep II tag inserted in loop 3

• 3] Codon optimized with E-coli K-12 strain.

• 4] Inhibitor 1 grafted in loop 6

• 5] C and N inteins added for circularization

Source

Reverse Translation